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IPRMB其他离体器官灌注系统的概述

HSE—HA生理学系统是一系列应用广泛的完备产品，可用于整体动物生理学、立体或在体器官和组织灌注及组织浴槽研究。这些系统具有广泛的应用，包括传统的安全药理学、DMPK、毒理学、呼吸系统、心脏病学、分子生物学和生理学。每个系统都设计了标准的组件，再加一些其他配置以适合特殊研究的需要。。标注组件也意味着如果你是需要将现有设备配齐，或者进行系统升级，或者是完整的配套产品，你都只需要购买需要的构件。HSE-HA生理学系统为你提供各种工具，使你的研究走向多产和成功。

我们提供大多数生理学检测设备，包括放大器、电极、传感器和电化学检测器。参数包括力、位移、压力、液体和气体流、心电图（单导联—12导统）、MAP（单个或多个部位）、脑电图、*、连续支流灌注传统的血气参数和电解质的分析，可以选择程序化软件和传统的刺激器作为放大器、电极和传感器等产品的补充。

HSE-DAQ系统产品家族（BDAS、ACAD、HAEMODYN、ISOHEART、PULMODYN、PENNOCK）可以记录你的初始信号，计算到处的参数，注释数据，快速简易的输入确定的数据用于分析。同时还可以选择用于进一步分析的软件模块，带状记录纸打印，自动和控制模块等。产品经过预先设置，打开包装即可使用，并专门设计符合你的系统配置和数据分析要求。

• For investigating the tone of small blood vessels under the effect of vasoactive substances

• Compact arrangement

• Organ is in warm and moist environment during perfusion

• Electrical field stimulation capabilities

The rat hind leg and the rabbit ear are conventional preparations for investigating the tone of blood vessels under the effect of vasoactive substances in the peripheral vascular bed, either at constant perfusion pressure or at constant flow. Both set-ups suffer from the disadvantage that the measurement is affected by early edema formation. In the rat, the mesenteric circulation receives approximay 20% of the cardiac output; its regulation may therefore contribute significantly to the systemic blood pressure and circulating blood volume. The presence of arterioles, i.e. the most important vessels in the regulation of vascular resistance, renders the mesenteric bed an important preparation for the study of vascular physiology, pathophysiology and pharmacology.

The organ bath for the rat mesenteric bed consists of a moist chamber with cover, both water jacketed. The mesenteric tissue is placed into the moist chamber on a stainless steel mesh which also acts as anode during

electrical stimulation. The superior mesenteric artery is cannulated （steel cannula） and perfused at constant flow （e.g. 5 ml/min） through a roller pump via a heat exchanger included in the moist chamber. The test drug is introduced through a separate roller pump or a syringe pump directly before the vascular bed preparation. The perfusion pressure is measured in a branch before the stainless steel cannula. For electrical stimulation, the Mesenteric Bed is placed on a stainless steel grid and a metal clip is attached to the aorta section near the steel cannula.

References:

1） Cusma-Pelogia, N. et al.: Endothelium Inactivation in In Vitro Perfused Vascular Beds - Comparison of Methods. J. Pharmacol and Toxicol. Methods 29, 157-163 （1993）

2） Potenza, M.A. et al.:Vasodialation induced by Capsaicin in Rat Mesenteric Vessels is probably independent of Nitric Oxide Synthesis. Pharm. Res. 30, 253 （1994）